| 质粒名称: | lentiCRISPR v2-sgControl |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 125836 |
| 存储实验室: | Boyi Gan |
|
载体骨架基本信息 |
|
| 载体名称: | LentiCRISPR V2 |
| 载体出品公司: | Feng Zhang |
| 载体类型: | 慢病毒表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素，100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Control |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 25 bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知（如果被销毁，则未知） |
|
文献引用方法 |
|
| 材料和方法部分: |
lentiCRISPR v2-sgControl was a gift from Boyi Gan (Addgene plasmid # 125836 ; http://n2t.net/addgene:125836 ; RRID:Addgene_125836)
|
| 参考文献部分: | BAP1 links metabolic regulation of ferroptosis to tumour suppression. Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, Li W, Gan B. Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 10.1038/s41556-018-0178-0. Epub 2018 Sep 10. 10.1038/s41556-018-0178-0 PubMed 30202049 |
买家导航
lentiCRISPR v2-sgControl载体质粒基本信息
lentiCRISPR v2-sgControl质粒图谱载体图谱和lentiCRISPR v2-sgControl载体序列质粒序列多克隆位点信息
lentiCRISPR v2-sgControl质粒载体简介
lentiCRISPR v2-sgControl质粒序列载体序列
LOCUS lentiCRISPR v2-sgControl 13013 bp DNA circular SYN 15-DEC-2025
DEFINITION KO control .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13013)
AUTHORS Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X,
Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P,
Li W, Gan B
TITLE BAP1 links metabolic regulation of ferroptosis to tumour
suppression.
JOURNAL Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi:
10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
PUBMED 30202049
REFERENCE 2 (bases 1 to 13013)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41556-018-0178-0"; journalName: "Nat Cell Biol"; date:
"2018-10"; volume: "20"; issue: "10"; pages: "1181-1192"
COMMENT Sequence Label: lentiCRISPR v2-sgControl
FEATURES Location/Qualifiers
source 1..13013
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2608..2856
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 2608..2628
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2779..2798
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 2878..2953
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 3015..3226
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 3245..3254
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3251..7354
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 3251..7354
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7355..7402
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 7403..7426
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 7436..7492
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 7493..8089
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 7986..8006
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 8105..8693
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8158..8178)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8576..8587)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8765..8998
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9024..9041)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9030..9254
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9300..9728
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9387..9406)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9597..9618
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9737..9757)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9742..10071
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9922..10057
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9984..10003
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10119..10166
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10185..10559
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10689..10810
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10726..10745)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10780..10799
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10859..10875)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10859..10875)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10872..10894)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10883..10899
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(10907..10913,10914..10931,10932..10937))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10952..10973
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11090..11107)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11261..11849)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11341..11360)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(12020..12811,12812..12880))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12643..12662
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12881..12985)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
2821 ttggctttat atatcttgtg gaaaggacga aacaccggca ctaccagagc taactcagtt
2881 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
2941 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
3001 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
3061 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
3121 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
3181 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
3241 cgctgccacc atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg
3301 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
3361 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
3421 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
3481 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
3541 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
3601 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
3661 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
3721 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
3781 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
3841 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
3901 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
3961 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
4021 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
4081 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
4141 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
4201 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
4261 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
4321 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
4381 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
4441 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
4501 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
4561 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
4621 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
4681 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
4741 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
4801 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
4861 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
4921 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
4981 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
5041 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
5101 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
5161 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
5221 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
5281 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
5341 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
5401 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
5461 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
5521 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
5581 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
5641 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
5701 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
5761 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
5821 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
5881 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
5941 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
6001 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
6061 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
6121 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
6181 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
6241 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
6301 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
6361 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
6421 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
6481 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
6541 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
6601 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
6661 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
6721 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
6781 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
6841 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
6901 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
6961 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
7021 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
7081 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
7141 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
7201 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
7261 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
7321 gacacggatc gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc
7381 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac
7441 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgaccgagta
7501 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
7561 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
7621 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
7681 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
7741 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
7801 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
7861 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
7921 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
7981 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
8041 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
8101 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
8161 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
8221 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
8281 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
8341 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
8401 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
8461 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
8521 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
8581 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
8641 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
8701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
8761 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
8821 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
8881 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
8941 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
9001 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
9061 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
9121 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
9181 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
9241 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
9301 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
9361 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
9421 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
9481 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
9541 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
9601 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
9661 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
9721 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
9781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
9841 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
9901 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
9961 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
10021 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
10081 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
10141 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
10201 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
10261 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
10321 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
10381 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
10441 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
10501 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
10561 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
10621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
10681 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
10741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
10801 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
10861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
10921 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
10981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
11041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
11101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
11161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
11221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
11281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
11341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
11401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
11461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
11521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
11581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
11641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
11701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
11761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
11821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
11881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
11941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
12001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
12061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
12121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
12181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
12241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
12301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
12361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
12421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
12481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
12541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
12601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
12661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
12721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
12781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
12841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
12901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
12961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
lentiCRISPR v2-sgControl载体图谱质粒图谱pdf版和lentiCRISPR v2-sgControl载体序列质粒序列等相关资料下载
lentiCRISPR v2-sgControl质粒载体应用举例
- 上一篇：lentiCRISPR v2-sgSLC7A11/xCT-1
- 下一篇：没有了
